Overall research areas
Cardiac metabolism using Hyperpolarized 13C (HP13C)
Metabolism of breast cancer disease using HP13
Metabolism of diabetic kidneys using HP13C
Rapid metabolic imaging of HP13C

Jan Henrik Ardenkjær-Larsen
Group leader
DTU/GE/DRCMR

Metabolic imaging using HP13C
Hyperpolarization physics
Formulation of compounds for hyperpolarization

Andreas Clemmensen
DRCMR/KU

Peter Magnusson
DRCMR

Rapid metabolic imaging of HP13C
Cardiac metabolism using HP13C

Mette Hauge Lauritzen
Lund University

Measuring metabolism using HP13C

Abubakr Eldiridiri
DRCMR/DTU Elektro

Sascha Gude
DRCMR

Laboratory

Objectives:
The Hyperpolarization group aim for better understanding and diagnosis of major chronic diseases by means of hyperpolarization techniques. The group uses hyperpolarized 13C-based MR techniques (HP13C) for studying the cell metabolism characteristics of chronic diseases. Ongoing projects based on the HP13C-technique focus on in vivo studies of altered cell metabolism in ischemic heart disease and on quantification of altered metabolism in diabetic kidneys as well as in cancer. The group has further a strong interest in methods for rapid metabolic imaging of hyperpolarized compounds as well as in formulation of new metabolically active compounds that can be hyperpolarized and followed in vivo for studies of metabolic pathways currently not detectable.

Resources:
For HP13C-research the hyperpolarization group uses a 4.7 T pre-clinical Varian MR-scanner with dedicated RF-coils for the 13C-nucleus and with physiological monitoring equipment. For hyperpolarizing 13C the group has a HyperSense (Oxford Instruments).

Selected publications:

Magnusson, P. O. ; Jensen, P. R.; Dyrby, T. B. ; Karlsson, M.; Lerche, M. H.; Hanson, L. G.
3D metabolic ex vivo sample imaging of hyperpolarized compounds using a 3D single-shot RARE (3D SS-RARE) sequence, combining spectral RF selectivity with under-sampled spectral encoding at signal read-out
Proceedings of 5th hyperpolarization symposium, the Netherlands, 105, 2015

Asghar Butt, S., Søgaard, L. V., Ardenkjaer-Larsen, J. H., Lauritzen, M. H., Engelholm, L. H., Paulson, O. B., Mirza, A. O., Holck, S., Magnusson, P. & Akeson, P.
Monitoring mammary tumor progression and effect of tamoxifen treatment in MMTV-PymT using MRI and magnetic resonance spectroscopy with hyperpolarized [1-(13) C]pyruvate.
Magnetic resonance in medicine. 73, 1, p. 51-58, 2015.
| WebSearch |

Søgaard, L. V., Schilling, F., Janich, M. A., Menzel, M. I. & Ardenkjaer-Larsen, J. H.
In vivo measurement of apparent diffusion coefficients of hyperpolarized ¹³C-labeled metabolites.
N M R in Biomedicine (Online). 27, 5, p. 561-9, 2014.
| WebSearch |

Laustsen, C., Ostergaard, J. A., Lauritzen, M. H., Nørregaard, R., Bowen, S., Søgaard, L. V., Flyvbjerg, A., Pedersen, M. & Ardenkjaer-Larsen, J. H.
Assessment of early diabetic renal changes with hyperpolarised [1-(13) C]pyruvate.
Diabetes - Metabolism: Research and Reviews (Online). 29, 2, p. 125-9, 2013.
| WebSearch |

Lauritzen, M. H., Laustsen, C., Butt, S. A., Magnusson, P., Søgaard, L. V., Ardenkjaer-Larsen, J. H. & Akeson, P.
Enhancing the [13C]bicarbonate signal in cardiac hyperpolarized [1-13C]pyruvate MRS studies by infusion of glucose, insulin and potassium.
N M R in Biomedicine (Online). 26, 11, p. 1496-1500, 2013.
| WebSearch |

Laustsen C, Pileio G, Tayler MC, Brown LJ, Brown RC, Levitt MH, Ardenkjaer-Larsen JH.
Hyperpolarized singlet NMR on a small animal imaging system.
Magnetic Resonance in Medicine 2012;68(4):1262-5

Butt SA, Søgaard LV, Magnusson P, Lauritzen M, Laustsen C, Åkeson P, Ardenkjær-Larsen JH
Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized 13C Magnetic Resonance Spectroscopic Imaging.
Journal of Cerebral Blood Flow&Metabolism 2012;32(8):1508-14

Collaborators:

Dept. Of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet
Bartholin Institute, Copenhagen Biocenter
Department of Pathology, Copenhagen University Hospital Hvidovre
GE Healthcare
HYPERMAG Center for Hyperpolarized Magnetic Resonance, DTU Elektro